I have a ~300 GB zipped vcf file (.vcf.gz) which contains the genomes of about 700 dogs. I am only interested in a few of these dogs and I do not have enough space to unzip the whole file at this time, although I am in the process of getting a computer to do this. Is it possible to unzip only parts of the file to begin testing my scripts?
I am trying to a specific SNP at a position on a subset of the samples. I have tried using bcftools to no avail: (If anyone can identify what went wrong with that I would also really appreciate it. I created an empty file for the output (722g.990.SNP.INDEL.chrAll.vcf.bgz) but it returns the following error)
bcftools view -f PASS --threads 8 -r chr9:55252802-55252810 -o 722g.990.SNP.INDEL.chrAll.vcf.gz -O z 722g.990.SNP.INDEL.chrAll.vcf.bgz
The output type "722g.990.SNP.INDEL.chrAll.vcf.bgz" not recognised
I am planning on trying awk, but need to unzip the file first. Is it possible to partially unzip it so I can try this?
Double check your command line for bcftools view.
The error message 'The output type "something" is not recognized' is printed by bcftools when you specify an invalid value for the -O (upper-case O) command line option like this -O something. Based on the error message you are getting it seems that you might have put the file name there.
Check that you don't have your input and output file names the wrong way around in your command. Note that the -o (lower-case o) command line option specifies the output file name, and the file name at the end of the command line is the input file name.
Also, you write that you created an empty file for the output. You don't need to do that, bcftools will create the output file.
I don't have that much experience with bcftools but generically If you want to to use awk to manipulate a gzipped file you can pipe to it so as to only unzip the file as needed, you can also pipe the result directly through gzip so it too is compressed e.g.
gzip -cd largeFile.vcf.gz | awk '{ <some awk> }' | gzip -c > newfile.txt.gz
Also zcat is an alias for gzip -cd, -c is input/output to standard out, -d is decompress.
As a side note if you are trying to perform operations on just a part of a large file you may also find the excellent tool less useful it can be used to view your large file loading only the needed parts, the -S option is particularly useful for wide formats with many columns as it stops line wrapping, as is -N for showing line numbers.
less -S largefile.vcf.gz
quit the view with q and g takes you to the top of the file.
Related
Does anybody know the command to remove the header from a ppm file in Linux? I've tried this already
´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´
head -n 4 Example.ppm > header.txt
tail -n 5+ Example.ppm > body.bin
´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´´
It tells me that "Tail" could not be found.
Most ppm files use newlines in the header so your first command is fine. However, the rest of the file is binary, so:
head -n 4 Example.ppm > header.txt
filesize=$(wc -c header.txt)
dd if=Example.ppm of=body.bin bs=1 skip=$filesize
You should have /bin/tail if you have /bin/head; both are in the coreutils RPM package.
The format of a ppm(5) file (http://netpbm.sourceforge.net/doc/ppm.html) is awkward to use with the line-based head/tail/sed family. The documentation describes fields separated by whitespace that is not necessarily a line break.
You will need to: 1) Ignore comments from '#' to end of line; and 2) process the remainder one field (not column, not line) at a time. Using awk(1) could be an option here.
Check the documentation (http://netpbm.sourceforge.net/doc/directory.html) for a list of conversion programs. You may find one that converts the PPM file into a form better suited to whatever usage is your ultimate goal.
I'm trying to display the output of an AWS lambda that is being captured in a temporary text file, and I want to remove that file as I display its contents. Right now I'm doing:
... && cat output.json && rm output.json
Is there a clever way to combine those last two commands into one command? My goal is to make the full combined command string as short as possible.
For cases where
it is possible to control the name of the temporary text file.
If file is not used by other code
Possible to pass "/dev/stdout" as the.name of the output
Regarding portability: see stack exchange how portable ... /dev/stdout
POSIX 7 says they are extensions.
Base Definitions,
Section 2.1.1 Requirements:
The system may provide non-standard extensions. These are features not required by POSIX.1-2008 and may include, but are not limited to:
[...]
• Additional character special files with special properties (for example, /dev/stdin, /dev/stdout, and /dev/stderr)
Using the mandatory supported /dev/tty will force output into “current” terminal, making it impossible to pipe the output of the whole command into different program (or log file), or to use the program when there is no connected terminals (cron job, or other automation tools)
No, you cannot easily remove the lines of a file while displaying them. It would be highly inefficient as it would require removing characters from the beginning of a file each time you read a line. Current filesystems are pretty good at truncating lines at the end of a file, but not at the beginning.
A simple but extremely slow method would look like this:
while [ -s output.json ]
do
head -1 output.json
sed -i 1d output.json
done
While this algorithm is plain and simple, you should know that each time you remove the first line with sed -i 1d it will copy the whole content of the file but the first line into a temporary file, resulting in approximately 0.5*n² lines written in total (where n is the number of lines in your file).
In theory you could avoid this by do something like that:
while [ -s output.json ]
do
line=$(head -1 output.json)
printf -- '%s\n' "$line"
fallocate -c -o 0 -l $((${#len}+1)) output.json
done
But this does not account for variable newline characters (namely DOS-formatted newlines) and fallocate does not always work on xfs, among other issues.
Since you are trying to consume a file alongside its creation without leaving a trace of its existence on disk, you are essentially asking for a pipe functionality. In my opinion you should look into how your output.json file is produced and hopefully you can pipe it to a script of your own.
I made a shell script the purpose of which is to find files that don't contain a particular string, then display the first line that isn't empty or otherwise useless. My script works well in the console, but for some reason when I try to direct the output to a .txt file, it comes out empty.
Here's my script:
#!/bin/bash
# takes user input.
echo "Input substance:"
read substance
echo "Listing media without $substance:"
cd media
# finds names of files that don't feature the substance given, then puts them inside an array.
searchresult=($(grep -L "$substance" *))
# iterates the array and prints the first line of each - contains both the number and the medium name.
# however, some files start with "Microorganisms" and the actual number and name feature after several empty lines
# the script checks for that occurence - and prints the first line that doesnt match these criteria.
for i in "${searchresult[#]}"
do
grep -m 1 -v "Microorganisms\|^$" $i
done >> output.txt
I've tried moving the >>output.txt to right after the grep line inside the loop, tried switching >> to > and 2>&1, tried using tee. No go.
I'm honestly feeling utterly stuck as to what the issue could be. I'm sure there's something I'm missing, but I'm nowhere near good enough with this to notice. I would very much appreciate any help.
EDIT: Added files to better illustrate what I'm working with. Sample inputs I tried: Glucose, Yeast extract, Agar. Link to files [140kB] - the folder was unzipped beforehand.
The script was given full permissions to execute. I don't think the output is being rewritten because even if I don't iterate and just run a single line of the loop, the file is empty.
I am trying to iterate through every file in a specific directory (called sequences), and perform two functions on each file. I know that the functions (the 'blastp' and 'cat' lines) work, since I can run them on individual files. Ordinarily I would have a specific file name as the query, output, etc., but I'm trying to use a variable so the loop can work through many files.
(Disclaimer: I am new to coding.) I believe that I am running into serious problems with trying to use my file names within my functions. As it is, my code will execute, but it creates a bunch of extra unintended files. This is what I intend for my script to do:
Line 1: Iterate through every file in my "sequences" directory. (All of which end with ".fa", if that is helpful.)
Line 3: Recognize the filename as a variable. (I know, I know, I think I've done this horribly wrong.)
Line 4: Run the blastp function using the file name as the argument for the "query" flag, always use "database.faa" as the argument for the "db" flag, and output the result in a new file that is has the same name as the initial file, but with ".txt" at the end.
Line 5: Output parts of the output file from line 4 into a new file that has the same name as the initial file, but with "_top_hits.txt" at the end.
for sequence in ./sequences/{.,}*;
do
echo "$sequence";
blastp -query $sequence -db database.faa -out ${sequence}.txt -evalue 1e-10 -outfmt 7
cat ${sequence}.txt | awk '/hits found/{getline;print}' | grep -v "#">${sequence}_top_hits.txt
done
When I ran this code, it gave me six new files derived from each file in the directory (and they were all in the same directory - I'd prefer to have them all in their own folders. How can I do that?). They were all empty. Their suffixes were, ".txt", ".txt.txt", ".txt_top_hits.txt", "_top_hits.txt", "_top_hits.txt.txt", and "_top_hits.txt_top_hits.txt".
If I can provide any further information to clarify anything, please let me know.
If you're only interested in *.fa files I would limit your input to only those matching files like this:
for sequence in sequences/*.fa;
do
I can propose you the following improvements:
for fasta_file in ./sequences/*.fa # ";" is not necessary if you already have a new line for your "do"
do
# ${variable%something} is the part of $variable
# before the string "something"
# basename path/to/file is the name of the file
# without the full path
# $(some command) allows you to use the result of the command as a string
# Combining the above, we can form a string based on our fasta file
# This string can be useful to name stuff in a clean manner later
sequence_name=$(basename ${fasta_file%.fa})
echo ${sequence_name}
# Create a directory for the results for this sequence
# -p option avoids a failure in case the directory already exists
mkdir -p ${sequence_name}
# Define the name of the file for the results
# (including our previously created directory in its path)
blast_results=${sequence_name}/${sequence_name}_blast.txt
blastp -query ${fasta_file} -db database.faa \
-out ${blast_results} \
-evalue 1e-10 -outfmt 7
# Define a file name for the top hits
top_hits=${sequence_name}/${sequence_name}_top_hits.txt
# alternatively, using "%"
#top_hits=${blast_results%_blast.txt}_top_hits.txt
# No need to cat: awk can take a file as argument
awk '/hits found/{getline;print}' ${blast_results} \
| grep -v "#" > ${sequence_name}_top_hits.txt
done
I made more intermediate variables, with (hopefully) meaningful names.
I used \ to escape line ends and allow putting commands in several lines.
I hope this improves code readability.
I haven't tested. There may be typos.
You should be using *.fa if you only want files with a .fa ending. Additionally, if you want to redirect your output to new folders you need to create those directories somewhere using
mkdir 'folder_name'
then you need to redirect your -o outputs to those files, something like this
'command' -o /path/to/output/folder
To help you test this script out, you can run each line one by one to test them. You need to make sure each line works by itself before combining.
One last thing, be careful with your use of colons, it should look something like this:
for filename in *.fa; do 'command'; done
I have large file with size of 130GB.
# ls -lrth
-rw-------. 1 root root 129G Apr 20 04:25 syslog.log
So I need to reduce file size by deleting line which starts with "Nov 2" , So I have given the following command,
sed -i '/Nov 2/d' syslog.log
So I can't edit file using VIM editor also.
When I trigger SED command , its creating backup file also. But I don't have much space in root. Please try to give alternate solution to delete particular line from this file without increasing space in server.
It does not create a real backup file. sed is a stream editor. When applied to a file with option -i it will stream that file through the sed process, write the output to a new file (a temporary one), when everything is done, it will rename the new file to the original name.
(There are options to create backup files also, but you didn't give them, so I won't mention that further.)
In your case you have a very large file and don't want to create any copy, however temporary. For this you need to open the file for reading and writing at the same time, then your sed process can overwrite the original. After this, you will have to truncate the file at the end of the writing.
To demonstrate how this can be done, we first perform a test case.
Create a test file, containing lots of lines:
seq 0 999999 > x
Now, lets say we want to remove all lines containing the digit 4:
grep -v 4 1<>x <x
This will open the file for reading and writing as STDOUT (1), and for reading as STDIN. The grep command will read all lines and will output only the lines not containing a 4 (option -v).
This will effectively overwrite the beginning of the original file.
You will not know how long the output is, so after the output the original contents of the file will appear:
…
999991
999992
999993
999995
999996
999997
999998
999999
537824
537825
537826
537827
537828
537829
…
You can use the Unix tool truncate to shorten your file manually afterwards. In a real scenario you will have trouble finding the right spot for this, so it makes sense to count the number of bytes written (using wc):
(Don't forget to recreate the original x for this test.)
(grep -v 4 <x | tee /dev/stderr 1<>x) |& wc -c
This will preform the step above and additionally print out the number of bytes written to the terminal, in this example case the output will be 3653658. Now use truncate:
truncate -s 3653658 x
Now you have the result you want.
If you want to do this in a script, i. e. without interaction, you can use this:
length=$((grep -v 4 <x | tee /dev/stderr 1<>x) |& wc -c)
truncate -s "$length" x
I cannot guarantee that this will work for files >2GB or >4GB on your machine; depending on your operating system (32bit?) and the versions of the installed tools you might run into largefile issues. I'd perform tests with large files first (>4GB as this is typically a limit for many things) and then cross your fingers and give it a try :)
Some caveats you have to keep in mind:
Of course, nobody is supposed to append log entries to that log file while the procedure is running.
Also, any abort during the running of the process (power failure, signal caught, etc.) will leave the file in an undefined state. But re-running the command again after such a mishap will in most cases produce the correct output; some lines might be doubled, but not more than a single line should be corrupted then.
The output must be smaller than the input, of course, otherwise the writing will overtake the reading, corrupting the whole result so that lines which should be there will be missing (or truncated at the start).